| AGT

Angiotensinogen is an alpha-2-globulin synthesized in the liver that is a precursor to angiotensin, but has also been noted to have many other effects unrelated to angiotensin peptides.It is a member of the serotonin family and has another name: serum protein A8, but it is not known to inhibit other enzymes like most serotonins.Angiotensinogen is also known as a renin substrate.It is cleaved by renin at the N-terminus to produce angiotensin I, which is subsequently modified to angiotensin II. The peptide is 485 amino acids long, and when renin acts on it, the N-terminal 10 amino acids are cleaved.Plasma angiotensinogen levels are increased by plasma corticosterone, estrogen, thyroid hormone, and angiotensin II levels.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	500	250	125	62.5	31.2	15.6	7.8	0
OD	2.22	1.56	0.92	0.63	0.42	0.25	0.18	0.01
Corrected OD	2.14	1.48	0.84	0.55	0.34	0.17	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse AGT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-106	103
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse AGT and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-95	90-110
	Average (%)	91	92	96
1 : 4	Range (%)	89-103	87-108	105-114
	Average (%)	94	98	107